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serum free cryopreservation medium  (Beyotime)


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    Beyotime serum free cryopreservation medium
    Serum Free Cryopreservation Medium, supplied by Beyotime, used in various techniques. Bioz Stars score: 99/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cell+freezing+medium/BeyoAOF+Serum-free+Cell+Freezing+Medium/pm42887604-47-10-26
    Average 99 stars, based on 2 article reviews
    serum free cryopreservation medium - by Bioz Stars, 2026-10
    99/100 stars

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    Related Articles

    Western Blot:

    Article Title: The miR-3164/PAD4 axis regulates NETosis to prevent airway inflammation and remodeling through the TLR2/NF-κB signaling pathway
    Article Snippet: Fetal bovine serum (FBS) was purchased from Excell Bio (Suzhou, China). .. Penicillin − streptomycin solution, cell freezing medium, phosphate buffer solution (PBS), Western cell lysate, IP cell lysate, APS, RIPA, PMSF, TEMED, an Annexin V-FITC apoptosis detection kit, a cell proliferation kit (MTT), 4% polyformaldehyde, a dual luciferase reporter gene detection kit, and a human TNF-α ELISA kit were purchased from Beyotime Biotechnology (Shanghai, China). .. Dulbecco’s modified Eagle’s medium and trypsin were purchased from Gibco (Shanghai, China).

    Article Title: <scp>ACSL3</scp>/<scp>GABARAPL2</scp> Ameliorates Vascular Endothelial Cell Aging and Injury Through Protective Autophagy to Alleviate Ferroptosis
    Article Snippet: Vascular endothelial cell (VEC) dysfunction is a key contributor to vascular diseases.. Oxidative stressinduced ferroptosis accelerates VEC aging and injury, while protective autophagy mitigates this damage by clearing damaged organelles and reducing oxidative stress.. GABARAPL2, a critical autophagyrelated protein, and ACSL3, a regulator of lipid metabolism and ferroptosis, are emerging as interconnected mediators of cellular protection.

    Article Title: The miR-3164/PAD4 axis regulates NETosis to prevent airway inflammation and remodeling through the TLR2/NF-κB signaling pathway.
    Article Snippet: Fetal bovine serum (FBS) was purchased from Excell Bio (Suzhou, China). .. Penicillin − streptomycin solution, cell freezing medium, phosphate buffer solution (PBS), Western cell lysate, IP cell lysate, APS, RIPA, PMSF, TEMED, an Annexin V-FITC apoptosis detection kit, a cell proliferation kit (MTT), 4% polyformaldehyde, a dual luciferase reporter gene detection kit, and a human TNF-α ELISA kit were purchased from Beyotime Biotechnology (Shanghai, China). .. Dulbecco’s modified Eagle’s medium and trypsin were purchased from Gibco (Shanghai, China).

    Article Title: Involvement of miR-205-5p in mediating the development of nodular thyroid disease associated with coal worker’s pneumoconiosis via pulmonary extracellular vesicles
    Article Snippet: .. The reagents used in this study include: Fetal bovine serum (FSD500, Excell Bio, China), Penicillin-Streptomycin solution (100X, Beyotime, China), RPMI 1640 medium (Gibco, China), Trypsin (Gibco, China), Cell freezing medium (C0210B, Beyotime, China), PBS buffer (C0221A, Beyotime, China), LipofectamineTM 3000 (Thermo Fisher, USA), Lentivirus (EnvirusTM, Beijing Yinggen Bio), RNA extraction and cDNA synthesis kits (Vazyme, China), Taq Pro qPCR Master Mix (Q712, Vazyme, China), BCA Protein Assay Kit (WB6501, NCM Biotech, China), Methanol (HB05, Guangzhou Chemical Reagent, China), and various antibodies (such as Anti-BAX, Anti-caspase-3, Anti-ATF4, Anti-CHOP from Abcam, USA).The instruments used include: Ultra-low temperature freezer (Haier), Optical microscope (Shanghai Optical Instrument Factory), CO2 incubator, Laminar flow cabinet (Shanghai Boxun Industrial & Medical Equipment Factory), Real-time quantitative PCR system (CFX96 Touch, Bio-Rad, USA), Western blotting system (CriterionTM Cell and Trans-Blot ® Cell, Bio-Rad, USA), Standard PCR machine (BIOER, China), EVOS M5000 Fluorescence Microscope (Thermo Fisher, USA), Shaker (Kylin-Bell, China), Chemiluminescence Imaging System (Shanghai JiaPeng), and Flow Cytometer (Thermo Fisher, USA). ..

    MTT Assay:

    Article Title: The miR-3164/PAD4 axis regulates NETosis to prevent airway inflammation and remodeling through the TLR2/NF-κB signaling pathway
    Article Snippet: Fetal bovine serum (FBS) was purchased from Excell Bio (Suzhou, China). .. Penicillin − streptomycin solution, cell freezing medium, phosphate buffer solution (PBS), Western cell lysate, IP cell lysate, APS, RIPA, PMSF, TEMED, an Annexin V-FITC apoptosis detection kit, a cell proliferation kit (MTT), 4% polyformaldehyde, a dual luciferase reporter gene detection kit, and a human TNF-α ELISA kit were purchased from Beyotime Biotechnology (Shanghai, China). .. Dulbecco’s modified Eagle’s medium and trypsin were purchased from Gibco (Shanghai, China).

    Article Title: The miR-3164/PAD4 axis regulates NETosis to prevent airway inflammation and remodeling through the TLR2/NF-κB signaling pathway.
    Article Snippet: Fetal bovine serum (FBS) was purchased from Excell Bio (Suzhou, China). .. Penicillin − streptomycin solution, cell freezing medium, phosphate buffer solution (PBS), Western cell lysate, IP cell lysate, APS, RIPA, PMSF, TEMED, an Annexin V-FITC apoptosis detection kit, a cell proliferation kit (MTT), 4% polyformaldehyde, a dual luciferase reporter gene detection kit, and a human TNF-α ELISA kit were purchased from Beyotime Biotechnology (Shanghai, China). .. Dulbecco’s modified Eagle’s medium and trypsin were purchased from Gibco (Shanghai, China).

    Luciferase:

    Article Title: The miR-3164/PAD4 axis regulates NETosis to prevent airway inflammation and remodeling through the TLR2/NF-κB signaling pathway
    Article Snippet: Fetal bovine serum (FBS) was purchased from Excell Bio (Suzhou, China). .. Penicillin − streptomycin solution, cell freezing medium, phosphate buffer solution (PBS), Western cell lysate, IP cell lysate, APS, RIPA, PMSF, TEMED, an Annexin V-FITC apoptosis detection kit, a cell proliferation kit (MTT), 4% polyformaldehyde, a dual luciferase reporter gene detection kit, and a human TNF-α ELISA kit were purchased from Beyotime Biotechnology (Shanghai, China). .. Dulbecco’s modified Eagle’s medium and trypsin were purchased from Gibco (Shanghai, China).

    Article Title: The miR-3164/PAD4 axis regulates NETosis to prevent airway inflammation and remodeling through the TLR2/NF-κB signaling pathway.
    Article Snippet: Fetal bovine serum (FBS) was purchased from Excell Bio (Suzhou, China). .. Penicillin − streptomycin solution, cell freezing medium, phosphate buffer solution (PBS), Western cell lysate, IP cell lysate, APS, RIPA, PMSF, TEMED, an Annexin V-FITC apoptosis detection kit, a cell proliferation kit (MTT), 4% polyformaldehyde, a dual luciferase reporter gene detection kit, and a human TNF-α ELISA kit were purchased from Beyotime Biotechnology (Shanghai, China). .. Dulbecco’s modified Eagle’s medium and trypsin were purchased from Gibco (Shanghai, China).

    Enzyme-linked Immunosorbent Assay:

    Article Title: The miR-3164/PAD4 axis regulates NETosis to prevent airway inflammation and remodeling through the TLR2/NF-κB signaling pathway
    Article Snippet: Fetal bovine serum (FBS) was purchased from Excell Bio (Suzhou, China). .. Penicillin − streptomycin solution, cell freezing medium, phosphate buffer solution (PBS), Western cell lysate, IP cell lysate, APS, RIPA, PMSF, TEMED, an Annexin V-FITC apoptosis detection kit, a cell proliferation kit (MTT), 4% polyformaldehyde, a dual luciferase reporter gene detection kit, and a human TNF-α ELISA kit were purchased from Beyotime Biotechnology (Shanghai, China). .. Dulbecco’s modified Eagle’s medium and trypsin were purchased from Gibco (Shanghai, China).

    Article Title: The miR-3164/PAD4 axis regulates NETosis to prevent airway inflammation and remodeling through the TLR2/NF-κB signaling pathway.
    Article Snippet: Fetal bovine serum (FBS) was purchased from Excell Bio (Suzhou, China). .. Penicillin − streptomycin solution, cell freezing medium, phosphate buffer solution (PBS), Western cell lysate, IP cell lysate, APS, RIPA, PMSF, TEMED, an Annexin V-FITC apoptosis detection kit, a cell proliferation kit (MTT), 4% polyformaldehyde, a dual luciferase reporter gene detection kit, and a human TNF-α ELISA kit were purchased from Beyotime Biotechnology (Shanghai, China). .. Dulbecco’s modified Eagle’s medium and trypsin were purchased from Gibco (Shanghai, China).

    Staining:

    Article Title: <scp>ACSL3</scp>/<scp>GABARAPL2</scp> Ameliorates Vascular Endothelial Cell Aging and Injury Through Protective Autophagy to Alleviate Ferroptosis
    Article Snippet: Vascular endothelial cell (VEC) dysfunction is a key contributor to vascular diseases.. Oxidative stressinduced ferroptosis accelerates VEC aging and injury, while protective autophagy mitigates this damage by clearing damaged organelles and reducing oxidative stress.. GABARAPL2, a critical autophagyrelated protein, and ACSL3, a regulator of lipid metabolism and ferroptosis, are emerging as interconnected mediators of cellular protection.

    Membrane:

    Article Title: <scp>ACSL3</scp>/<scp>GABARAPL2</scp> Ameliorates Vascular Endothelial Cell Aging and Injury Through Protective Autophagy to Alleviate Ferroptosis
    Article Snippet: Vascular endothelial cell (VEC) dysfunction is a key contributor to vascular diseases.. Oxidative stressinduced ferroptosis accelerates VEC aging and injury, while protective autophagy mitigates this damage by clearing damaged organelles and reducing oxidative stress.. GABARAPL2, a critical autophagyrelated protein, and ACSL3, a regulator of lipid metabolism and ferroptosis, are emerging as interconnected mediators of cellular protection.

    other:

    Article Title: Multi-omics reveals molecular basis of beef quality differences between Qinchuan and Nanyang cattle
    Article Snippet: The supernatant was discarded, and the pellet was resuspended in cell freezing medium (Beyotime Biotechnology, Shanghai, China).

    Sterility:

    Article Title: CYP27A1 suppresses brain metastasis via ferroptosis in lung adenocarcinoma: a six-gene signature predicting the immunotherapy response and clinical outcomes
    Article Snippet: For subculture, cells were seeded at 3 × 105 cells/well in 25T flasks (Corning, 430168) and passaged every 48 h using 0.25% trypsin-EDTA (Gibco, 25200056) upon reaching 90% confluency. .. The key reagents used included cell freezing medium (Beyotime, C0210), PBS (Gibco, 10010023), and sterile pipette tips (Nanjing Yifeixue Biotechnology Co., Ltd.). .. The full-length cDNA of CYP27A1 was subsequently cloned and inserted into the PCDH-CMV-GFP-Puro vector (NovoPro, V006738) via homologous recombination.

    Article Title: CYP27A1 suppresses brain metastasis via ferroptosis in lung adenocarcinoma: a six-gene signature predicting the immunotherapy response and clinical outcomes.
    Article Snippet: For subculture, cells were seeded at 3×105 cells/well in 25T flasks (Corning, 430168) and passaged every 48 h using 0.25% trypsin-EDTA (Gibco, 25200056) upon reaching 90% confluency. .. The key reagents used included cell freezing medium (Beyotime, C0210), PBS (Gibco, 10010023), and sterile pipette tips (Nanjing Yifeixue Biotechnology Co., Ltd.). .. The full-length cDNA of CYP27A1 was subsequently cloned and inserted into the PCDH-CMV-GFP-Puro vector (NovoPro, V006738) via homologous recombination.

    Transferring:

    Article Title: CYP27A1 suppresses brain metastasis via ferroptosis in lung adenocarcinoma: a six-gene signature predicting the immunotherapy response and clinical outcomes
    Article Snippet: For subculture, cells were seeded at 3 × 105 cells/well in 25T flasks (Corning, 430168) and passaged every 48 h using 0.25% trypsin-EDTA (Gibco, 25200056) upon reaching 90% confluency. .. The key reagents used included cell freezing medium (Beyotime, C0210), PBS (Gibco, 10010023), and sterile pipette tips (Nanjing Yifeixue Biotechnology Co., Ltd.). .. The full-length cDNA of CYP27A1 was subsequently cloned and inserted into the PCDH-CMV-GFP-Puro vector (NovoPro, V006738) via homologous recombination.

    Article Title: CYP27A1 suppresses brain metastasis via ferroptosis in lung adenocarcinoma: a six-gene signature predicting the immunotherapy response and clinical outcomes.
    Article Snippet: For subculture, cells were seeded at 3×105 cells/well in 25T flasks (Corning, 430168) and passaged every 48 h using 0.25% trypsin-EDTA (Gibco, 25200056) upon reaching 90% confluency. .. The key reagents used included cell freezing medium (Beyotime, C0210), PBS (Gibco, 10010023), and sterile pipette tips (Nanjing Yifeixue Biotechnology Co., Ltd.). .. The full-length cDNA of CYP27A1 was subsequently cloned and inserted into the PCDH-CMV-GFP-Puro vector (NovoPro, V006738) via homologous recombination.

    RNA Extraction:

    Article Title: Involvement of miR-205-5p in mediating the development of nodular thyroid disease associated with coal worker’s pneumoconiosis via pulmonary extracellular vesicles
    Article Snippet: .. The reagents used in this study include: Fetal bovine serum (FSD500, Excell Bio, China), Penicillin-Streptomycin solution (100X, Beyotime, China), RPMI 1640 medium (Gibco, China), Trypsin (Gibco, China), Cell freezing medium (C0210B, Beyotime, China), PBS buffer (C0221A, Beyotime, China), LipofectamineTM 3000 (Thermo Fisher, USA), Lentivirus (EnvirusTM, Beijing Yinggen Bio), RNA extraction and cDNA synthesis kits (Vazyme, China), Taq Pro qPCR Master Mix (Q712, Vazyme, China), BCA Protein Assay Kit (WB6501, NCM Biotech, China), Methanol (HB05, Guangzhou Chemical Reagent, China), and various antibodies (such as Anti-BAX, Anti-caspase-3, Anti-ATF4, Anti-CHOP from Abcam, USA).The instruments used include: Ultra-low temperature freezer (Haier), Optical microscope (Shanghai Optical Instrument Factory), CO2 incubator, Laminar flow cabinet (Shanghai Boxun Industrial & Medical Equipment Factory), Real-time quantitative PCR system (CFX96 Touch, Bio-Rad, USA), Western blotting system (CriterionTM Cell and Trans-Blot ® Cell, Bio-Rad, USA), Standard PCR machine (BIOER, China), EVOS M5000 Fluorescence Microscope (Thermo Fisher, USA), Shaker (Kylin-Bell, China), Chemiluminescence Imaging System (Shanghai JiaPeng), and Flow Cytometer (Thermo Fisher, USA). ..

    cDNA Synthesis:

    Article Title: Involvement of miR-205-5p in mediating the development of nodular thyroid disease associated with coal worker’s pneumoconiosis via pulmonary extracellular vesicles
    Article Snippet: .. The reagents used in this study include: Fetal bovine serum (FSD500, Excell Bio, China), Penicillin-Streptomycin solution (100X, Beyotime, China), RPMI 1640 medium (Gibco, China), Trypsin (Gibco, China), Cell freezing medium (C0210B, Beyotime, China), PBS buffer (C0221A, Beyotime, China), LipofectamineTM 3000 (Thermo Fisher, USA), Lentivirus (EnvirusTM, Beijing Yinggen Bio), RNA extraction and cDNA synthesis kits (Vazyme, China), Taq Pro qPCR Master Mix (Q712, Vazyme, China), BCA Protein Assay Kit (WB6501, NCM Biotech, China), Methanol (HB05, Guangzhou Chemical Reagent, China), and various antibodies (such as Anti-BAX, Anti-caspase-3, Anti-ATF4, Anti-CHOP from Abcam, USA).The instruments used include: Ultra-low temperature freezer (Haier), Optical microscope (Shanghai Optical Instrument Factory), CO2 incubator, Laminar flow cabinet (Shanghai Boxun Industrial & Medical Equipment Factory), Real-time quantitative PCR system (CFX96 Touch, Bio-Rad, USA), Western blotting system (CriterionTM Cell and Trans-Blot ® Cell, Bio-Rad, USA), Standard PCR machine (BIOER, China), EVOS M5000 Fluorescence Microscope (Thermo Fisher, USA), Shaker (Kylin-Bell, China), Chemiluminescence Imaging System (Shanghai JiaPeng), and Flow Cytometer (Thermo Fisher, USA). ..

    Real-time Polymerase Chain Reaction:

    Article Title: Involvement of miR-205-5p in mediating the development of nodular thyroid disease associated with coal worker’s pneumoconiosis via pulmonary extracellular vesicles
    Article Snippet: .. The reagents used in this study include: Fetal bovine serum (FSD500, Excell Bio, China), Penicillin-Streptomycin solution (100X, Beyotime, China), RPMI 1640 medium (Gibco, China), Trypsin (Gibco, China), Cell freezing medium (C0210B, Beyotime, China), PBS buffer (C0221A, Beyotime, China), LipofectamineTM 3000 (Thermo Fisher, USA), Lentivirus (EnvirusTM, Beijing Yinggen Bio), RNA extraction and cDNA synthesis kits (Vazyme, China), Taq Pro qPCR Master Mix (Q712, Vazyme, China), BCA Protein Assay Kit (WB6501, NCM Biotech, China), Methanol (HB05, Guangzhou Chemical Reagent, China), and various antibodies (such as Anti-BAX, Anti-caspase-3, Anti-ATF4, Anti-CHOP from Abcam, USA).The instruments used include: Ultra-low temperature freezer (Haier), Optical microscope (Shanghai Optical Instrument Factory), CO2 incubator, Laminar flow cabinet (Shanghai Boxun Industrial & Medical Equipment Factory), Real-time quantitative PCR system (CFX96 Touch, Bio-Rad, USA), Western blotting system (CriterionTM Cell and Trans-Blot ® Cell, Bio-Rad, USA), Standard PCR machine (BIOER, China), EVOS M5000 Fluorescence Microscope (Thermo Fisher, USA), Shaker (Kylin-Bell, China), Chemiluminescence Imaging System (Shanghai JiaPeng), and Flow Cytometer (Thermo Fisher, USA). ..

    Bicinchoninic Acid Protein Assay:

    Article Title: Involvement of miR-205-5p in mediating the development of nodular thyroid disease associated with coal worker’s pneumoconiosis via pulmonary extracellular vesicles
    Article Snippet: .. The reagents used in this study include: Fetal bovine serum (FSD500, Excell Bio, China), Penicillin-Streptomycin solution (100X, Beyotime, China), RPMI 1640 medium (Gibco, China), Trypsin (Gibco, China), Cell freezing medium (C0210B, Beyotime, China), PBS buffer (C0221A, Beyotime, China), LipofectamineTM 3000 (Thermo Fisher, USA), Lentivirus (EnvirusTM, Beijing Yinggen Bio), RNA extraction and cDNA synthesis kits (Vazyme, China), Taq Pro qPCR Master Mix (Q712, Vazyme, China), BCA Protein Assay Kit (WB6501, NCM Biotech, China), Methanol (HB05, Guangzhou Chemical Reagent, China), and various antibodies (such as Anti-BAX, Anti-caspase-3, Anti-ATF4, Anti-CHOP from Abcam, USA).The instruments used include: Ultra-low temperature freezer (Haier), Optical microscope (Shanghai Optical Instrument Factory), CO2 incubator, Laminar flow cabinet (Shanghai Boxun Industrial & Medical Equipment Factory), Real-time quantitative PCR system (CFX96 Touch, Bio-Rad, USA), Western blotting system (CriterionTM Cell and Trans-Blot ® Cell, Bio-Rad, USA), Standard PCR machine (BIOER, China), EVOS M5000 Fluorescence Microscope (Thermo Fisher, USA), Shaker (Kylin-Bell, China), Chemiluminescence Imaging System (Shanghai JiaPeng), and Flow Cytometer (Thermo Fisher, USA). ..

    Microscopy:

    Article Title: Involvement of miR-205-5p in mediating the development of nodular thyroid disease associated with coal worker’s pneumoconiosis via pulmonary extracellular vesicles
    Article Snippet: .. The reagents used in this study include: Fetal bovine serum (FSD500, Excell Bio, China), Penicillin-Streptomycin solution (100X, Beyotime, China), RPMI 1640 medium (Gibco, China), Trypsin (Gibco, China), Cell freezing medium (C0210B, Beyotime, China), PBS buffer (C0221A, Beyotime, China), LipofectamineTM 3000 (Thermo Fisher, USA), Lentivirus (EnvirusTM, Beijing Yinggen Bio), RNA extraction and cDNA synthesis kits (Vazyme, China), Taq Pro qPCR Master Mix (Q712, Vazyme, China), BCA Protein Assay Kit (WB6501, NCM Biotech, China), Methanol (HB05, Guangzhou Chemical Reagent, China), and various antibodies (such as Anti-BAX, Anti-caspase-3, Anti-ATF4, Anti-CHOP from Abcam, USA).The instruments used include: Ultra-low temperature freezer (Haier), Optical microscope (Shanghai Optical Instrument Factory), CO2 incubator, Laminar flow cabinet (Shanghai Boxun Industrial & Medical Equipment Factory), Real-time quantitative PCR system (CFX96 Touch, Bio-Rad, USA), Western blotting system (CriterionTM Cell and Trans-Blot ® Cell, Bio-Rad, USA), Standard PCR machine (BIOER, China), EVOS M5000 Fluorescence Microscope (Thermo Fisher, USA), Shaker (Kylin-Bell, China), Chemiluminescence Imaging System (Shanghai JiaPeng), and Flow Cytometer (Thermo Fisher, USA). ..

    Polymerase Chain Reaction:

    Article Title: Involvement of miR-205-5p in mediating the development of nodular thyroid disease associated with coal worker’s pneumoconiosis via pulmonary extracellular vesicles
    Article Snippet: .. The reagents used in this study include: Fetal bovine serum (FSD500, Excell Bio, China), Penicillin-Streptomycin solution (100X, Beyotime, China), RPMI 1640 medium (Gibco, China), Trypsin (Gibco, China), Cell freezing medium (C0210B, Beyotime, China), PBS buffer (C0221A, Beyotime, China), LipofectamineTM 3000 (Thermo Fisher, USA), Lentivirus (EnvirusTM, Beijing Yinggen Bio), RNA extraction and cDNA synthesis kits (Vazyme, China), Taq Pro qPCR Master Mix (Q712, Vazyme, China), BCA Protein Assay Kit (WB6501, NCM Biotech, China), Methanol (HB05, Guangzhou Chemical Reagent, China), and various antibodies (such as Anti-BAX, Anti-caspase-3, Anti-ATF4, Anti-CHOP from Abcam, USA).The instruments used include: Ultra-low temperature freezer (Haier), Optical microscope (Shanghai Optical Instrument Factory), CO2 incubator, Laminar flow cabinet (Shanghai Boxun Industrial & Medical Equipment Factory), Real-time quantitative PCR system (CFX96 Touch, Bio-Rad, USA), Western blotting system (CriterionTM Cell and Trans-Blot ® Cell, Bio-Rad, USA), Standard PCR machine (BIOER, China), EVOS M5000 Fluorescence Microscope (Thermo Fisher, USA), Shaker (Kylin-Bell, China), Chemiluminescence Imaging System (Shanghai JiaPeng), and Flow Cytometer (Thermo Fisher, USA). ..

    Fluorescence:

    Article Title: Involvement of miR-205-5p in mediating the development of nodular thyroid disease associated with coal worker’s pneumoconiosis via pulmonary extracellular vesicles
    Article Snippet: .. The reagents used in this study include: Fetal bovine serum (FSD500, Excell Bio, China), Penicillin-Streptomycin solution (100X, Beyotime, China), RPMI 1640 medium (Gibco, China), Trypsin (Gibco, China), Cell freezing medium (C0210B, Beyotime, China), PBS buffer (C0221A, Beyotime, China), LipofectamineTM 3000 (Thermo Fisher, USA), Lentivirus (EnvirusTM, Beijing Yinggen Bio), RNA extraction and cDNA synthesis kits (Vazyme, China), Taq Pro qPCR Master Mix (Q712, Vazyme, China), BCA Protein Assay Kit (WB6501, NCM Biotech, China), Methanol (HB05, Guangzhou Chemical Reagent, China), and various antibodies (such as Anti-BAX, Anti-caspase-3, Anti-ATF4, Anti-CHOP from Abcam, USA).The instruments used include: Ultra-low temperature freezer (Haier), Optical microscope (Shanghai Optical Instrument Factory), CO2 incubator, Laminar flow cabinet (Shanghai Boxun Industrial & Medical Equipment Factory), Real-time quantitative PCR system (CFX96 Touch, Bio-Rad, USA), Western blotting system (CriterionTM Cell and Trans-Blot ® Cell, Bio-Rad, USA), Standard PCR machine (BIOER, China), EVOS M5000 Fluorescence Microscope (Thermo Fisher, USA), Shaker (Kylin-Bell, China), Chemiluminescence Imaging System (Shanghai JiaPeng), and Flow Cytometer (Thermo Fisher, USA). ..

    Imaging:

    Article Title: Involvement of miR-205-5p in mediating the development of nodular thyroid disease associated with coal worker’s pneumoconiosis via pulmonary extracellular vesicles
    Article Snippet: .. The reagents used in this study include: Fetal bovine serum (FSD500, Excell Bio, China), Penicillin-Streptomycin solution (100X, Beyotime, China), RPMI 1640 medium (Gibco, China), Trypsin (Gibco, China), Cell freezing medium (C0210B, Beyotime, China), PBS buffer (C0221A, Beyotime, China), LipofectamineTM 3000 (Thermo Fisher, USA), Lentivirus (EnvirusTM, Beijing Yinggen Bio), RNA extraction and cDNA synthesis kits (Vazyme, China), Taq Pro qPCR Master Mix (Q712, Vazyme, China), BCA Protein Assay Kit (WB6501, NCM Biotech, China), Methanol (HB05, Guangzhou Chemical Reagent, China), and various antibodies (such as Anti-BAX, Anti-caspase-3, Anti-ATF4, Anti-CHOP from Abcam, USA).The instruments used include: Ultra-low temperature freezer (Haier), Optical microscope (Shanghai Optical Instrument Factory), CO2 incubator, Laminar flow cabinet (Shanghai Boxun Industrial & Medical Equipment Factory), Real-time quantitative PCR system (CFX96 Touch, Bio-Rad, USA), Western blotting system (CriterionTM Cell and Trans-Blot ® Cell, Bio-Rad, USA), Standard PCR machine (BIOER, China), EVOS M5000 Fluorescence Microscope (Thermo Fisher, USA), Shaker (Kylin-Bell, China), Chemiluminescence Imaging System (Shanghai JiaPeng), and Flow Cytometer (Thermo Fisher, USA). ..

    Flow Cytometry:

    Article Title: Involvement of miR-205-5p in mediating the development of nodular thyroid disease associated with coal worker’s pneumoconiosis via pulmonary extracellular vesicles
    Article Snippet: .. The reagents used in this study include: Fetal bovine serum (FSD500, Excell Bio, China), Penicillin-Streptomycin solution (100X, Beyotime, China), RPMI 1640 medium (Gibco, China), Trypsin (Gibco, China), Cell freezing medium (C0210B, Beyotime, China), PBS buffer (C0221A, Beyotime, China), LipofectamineTM 3000 (Thermo Fisher, USA), Lentivirus (EnvirusTM, Beijing Yinggen Bio), RNA extraction and cDNA synthesis kits (Vazyme, China), Taq Pro qPCR Master Mix (Q712, Vazyme, China), BCA Protein Assay Kit (WB6501, NCM Biotech, China), Methanol (HB05, Guangzhou Chemical Reagent, China), and various antibodies (such as Anti-BAX, Anti-caspase-3, Anti-ATF4, Anti-CHOP from Abcam, USA).The instruments used include: Ultra-low temperature freezer (Haier), Optical microscope (Shanghai Optical Instrument Factory), CO2 incubator, Laminar flow cabinet (Shanghai Boxun Industrial & Medical Equipment Factory), Real-time quantitative PCR system (CFX96 Touch, Bio-Rad, USA), Western blotting system (CriterionTM Cell and Trans-Blot ® Cell, Bio-Rad, USA), Standard PCR machine (BIOER, China), EVOS M5000 Fluorescence Microscope (Thermo Fisher, USA), Shaker (Kylin-Bell, China), Chemiluminescence Imaging System (Shanghai JiaPeng), and Flow Cytometer (Thermo Fisher, USA). ..

    LDH Cytotoxicity Assay:

    Article Title: Highly Efficient Bifunctional Peptides for Tumor Immunotherapy by Simultaneously Activating T Cells and Blocking PD-L1 Immune Checkpoint.
    Article Snippet: Immune checkpoint inhibitors represented by PD-1/PD-L1 monoclonal antibodies have shown great success in tumor immunotherapy.. However, the response rate of immune checkpoint blockade (ICB) therapy alone is far from satisfactory due to insufficient and exhausted tumor-infiltrating T cells.. Meanwhile, antibody-based drugs have some drawbacks such as high cost and complicated preparation, which require further development of nonantibody immune checkpoint inhibitors and more rational strategies for improving the effectiveness of tumor treatment.



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